Plasmid

Part:BBa_K1843000:Design

Designed by: Cristina Castillo   Group: iGEM15_Michigan   (2015-09-18)


Thrombin switch 1.0


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 31
    Illegal SpeI site found at 972
    Illegal PstI site found at 838
    Illegal PstI site found at 978
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 972
    Illegal PstI site found at 838
    Illegal PstI site found at 978
    Illegal NotI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 31
    Illegal SpeI site found at 972
    Illegal PstI site found at 838
    Illegal PstI site found at 978
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 31
    Illegal SpeI site found at 972
    Illegal PstI site found at 838
    Illegal PstI site found at 978
    Illegal AgeI site found at 189
    Illegal AgeI site found at 312
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ease the cloning process for future teams, an additional HindIII cut site was added between the switch and GFP. The HindIII cut site combined with the iGEM cut sites that surround the switch makes it easy to substitute the reporter.



Source

Synthesized based on published aptamer and GFP sequence

References